In cellulo serial crystallography of alcohol oxidase crystals inside yeast cells

Autor:	Arjen J. Jakobi, Daniel M. Passon, Kèvin Knoops, Francesco Stellato, Mengning Liang, Thomas A. White, Thomas Seine, Marc Messerschmidt, Henry N. Chapman, Matthias Wilmanns
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	protein structure X-ray crystallography femtosecond studies nanocrystals free-electron laser Crystallography QD901-999
Zdroj:	IUCrJ, Vol 3, Iss 2, Pp 88-95 (2016)
Druh dokumentu:	article
ISSN:	2052-2525 20522525
DOI:	10.1107/S2052252515022927
Popis:	The possibility of using femtosecond pulses from an X-ray free-electron laser to collect diffraction data from protein crystals formed in their native cellular organelle has been explored. X-ray diffraction of submicrometre-sized alcohol oxidase crystals formed in peroxisomes within cells of genetically modified variants of the methylotrophic yeast Hansenula polymorpha is reported and characterized. The observations are supported by synchrotron radiation-based powder diffraction data and electron microscopy. Based on these findings, the concept of in cellulo serial crystallography on protein targets imported into yeast peroxisomes without the need for protein purification as a requirement for subsequent crystallization is outlined.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/0e8bb1c71e8b4e489bc877f3c0bf30f4 Zobrazit plný text záznamu View record in DOAJ