| Autor: |
Rubén D. Caffarena, Matías Castells, Carlos O. Schild, María L. Casaux, Joaquín I. Armendano, Rodney Colina, Federico Giannitti |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Veterinary Science, Vol 9 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2297-1769 |
| DOI: |
10.3389/fvets.2022.952197 |
| Popis: |
Rotavirus A (RVA) is amongst the most widespread causes of neonatal calf diarrhea. Because subclinical infections are common, the diagnosis of RVA-induced diarrhea cannot rely solely on molecular viral detection. However, RT-qPCR allows for quantification of RVA shedding in feces, which can be correlated with clinical disease. Here, we determine an optimal cutoff of rotaviral load quantified by RT-qPCR to predict RVA causality in diarrheic neonate calves, using RVA antigen-capture ELISA as reference test. Feces from 328 diarrheic (n = 175) and non-diarrheic (n = 153), |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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