| Autor: |
Neera Yadav, Ashish Dwivedi, S. Faiz Mujtaba, Manish K. Pal, Gajendra Singh, Ratan Singh Ray |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
G-Journal of Environmental Science and Technology, Vol 1, Iss 6 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2322-0228 |
| Popis: |
Simultaneous and sufficient exposure to sunlight and drug cause phototoxic reactions in the skin. In the present study, the photosensitizing activity of quinine (Q) was studied. Evidences obtained by reduction of cell viability, mitochondrial damage, DNA damage, ROS generation, lysosome damage and phosphatidyl serine translocation as a marker of apoptosis indicates that exposure of quinine treated keratinocytes to UV-B radiation results in quinine photosensitization and subsequent reactions which alter normal cellular processes. Quinine absorbs strongly in UV-B region (330 nm). It reduced viability of cells significantly as observed through MTT assay. Reduction of cell viability was confirmed through accumulation of Rhodamine 123 dye which showed that quinine and UV-B treated cells accumulate less than control cells confirmed that mitochondrial membrane potential was disturbed. Increased intracellular ROS production was measured through DCF fluorescence. Early apoptosis was confirmed through phosphatidyl serine translocation in the membrane. Increased lysosome damage was observed through acridine orange accumulation in the lysosmes. Single stranded damage was confirmed through comet assay and apoptosis was confirmed through EB/AO staining. These results show that UV-B radiation elicits phototoxic effects in keratinocytes through reactive oxygen species generation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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