In vitro and in vivo evaluations of glass-ionomer cement containing chlorhexidine for Atraumatic Restorative Treatment

Autor: Cristiane Duque, Kelly Limi Aida, Jesse Augusto Pereira, Gláucia Schuindt Teixeira, Angela Scarparo Caldo-Teixeira, Luciana Rodrigues Perrone, Karina Sampaio Caiaffa, Thais de Cássia Negrini, Aline Rogéria Freire de Castilho, Carlos Alberto de Souza Costa
Jazyk: angličtina
Předmět:
Zdroj: Journal of Applied Oral Science, Vol 25, Iss 5, Pp 541-550
Druh dokumentu: article
ISSN: 1678-7765
1678-7757
DOI: 10.1590/1678-7757-2016-0195
Popis: Abstract Objectives: Addition of chlorhexidine has enhanced the antimicrobial effect of glass ionomer cement (GIC) indicated to Atraumatic Restorative Treatment (ART); however, the impact of this mixture on the properties of these materials and on the longevity of restorations must be investigated. The aim of this study was to evaluate the effects of incorporating chlorhexidine (CHX) in the in vitro biological and chemical-mechanical properties of GIC and in vivo clinical/ microbiological follow-up of the ART with GIC containing or not CHX. Material and Methods: For in vitro studies, groups were divided into GIC, GIC with 1.25% CHX, and GIC with 2.5% CHX. Antimicrobial activity of GIC was analyzed using agar diffusion and anti-biofilm assays. Cytotoxic effects, compressive tensile strength, microhardness and fluoride (F) release were also evaluated. A randomized controlled trial was conducted on 36 children that received ART either with GIC or GIC with CHX. Saliva and biofilm were collected for mutans streptococci (MS) counts and the survival rate of restorations was checked after 7 days, 3 months and one year after ART. ANOVA/Tukey or Kruskal-Wallis/ Mann-Whitney tests were performed for in vitro tests and in vivo microbiological analysis. The Kaplan-Meier method and Log rank tests were applied to estimate survival percentages of restorations (p
Databáze: Directory of Open Access Journals
Externí odkaz: