MiR-145 regulates steroidogenesis in mouse primary granulosa cells by targeting Arpc5 and subsequent cytoskeleton remodeling
| Autor: | Lanfang MA, Shuo WANG, Jun YANG, Weicheng TANG, Zhangying WU, Lili CAO, Aiyue LUO, Fangfang FU, Shuhong YANG, Shixuan WANG |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | The Journal of Reproduction and Development, Vol 69, Iss 3, Pp 154-162 (2023) |
| Druh dokumentu: | article |
| ISSN: | 0916-8818 1348-4400 |
| DOI: | 10.1262/jrd.2022-137 |
| Popis: | MicroRNA (miR)-145 is enriched in the follicular granulosa cells (GCs) of 3-week-old mice. Downregulating miR-145 inhibits the proliferation and differentiation of GCs and induces evident changes in their cytoskeleton. In this study, we examined how miR-145 induces cytoskeletal changes in mouse GCs and its potential mechanism in regulating GC steroidogenesis. We found that actin related protein 2/3 complex subunit 5 (Arpc5) is a target of miR-145. The miR-145 antagomir increased ARPC5 expression but not β-ACTIN, β-TUBULIN, and PAXILLIN expression. Arpc5 overexpression inhibited GC proliferation, differentiation, and progesterone synthesis. Furthermore, the expression of progesterone synthesis-associated enzymes was downregulated in the Arpc5 overexpression group, and the GC cytoskeleton exhibited evident changes. We conclude that Arpc5, a new target of miR-145, regulates primary GC proliferation and progesterone production by regulating the cytoskeleton remodeling. |
| Databáze: | Directory of Open Access Journals |
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