Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study

Autor:	Shirleny R Cardosa, B. William Ogunkolade, Rob Lowe, Emanuel Savage, Charles A Mein, Barbara J Boucher, Graham A Hitman
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Betel-nut Type 2 diabetes Obesity Transcriptomics RNA-sequencing Diseases of the endocrine glands. Clinical endocrinology RC648-665
Zdroj:	BMC Endocrine Disorders, Vol 21, Iss 1, Pp 1-11 (2021)
Druh dokumentu:	article
ISSN:	1472-6823
DOI:	10.1186/s12902-021-00827-1
Popis:	Abstract Background Betel-nut consumption is the fourth most common addictive habit globally and there is good evidence linking the habit to obesity, type 2 diabetes (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene expression relevant to obesity, T2D and the metabolic syndrome using a genome-wide transcriptomic approach in a human monocyte cell line incubated with arecoline and its nitrosated products. Results The THP1 monocyte cell line was incubated separately with arecoline and 3-methylnitrosaminopropionaldehyde (MNPA) in triplicate for 24 h and pooled cDNA indexed paired-end libraries were sequenced (Illumina NextSeq 500). After incubation with arecoline and MNPA, 15 and 39 genes respectively had significant changes in their expression (q
Databáze:	Directory of Open Access Journals
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