The Effects of Thymoquinone on Semen Quality in the Diazinon Exposed Rats
| Autor: | Lila Tajik, Sima Nasri, Ehsan Raeis-Abdollahi |
|---|---|
| Jazyk: | English<br />Persian |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Iranian South Medical Journal, Vol 23, Iss 5, Pp 505-514 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1735-4374 1735-6954 |
| Popis: | Background: Exposure to organophosphate pesticides, including diazinon, can lead to increased production of free radicals and decreased cellular antioxidant capacity and male infertility. Thymoquinone (TQ) is an active component of Nigella sativa with well documented antioxidant effects. This study evaluated the effects of thymoquinone on oxidative stress induced by exposure to diazinon and semen quality in male rats. Materials and Methods: Twenty-eight male Wistar rats weighing 200-300 g were randomly divided into four groups (n=7, each). The first (control) group received a single intraperitoneal (i.p.) injection of olive oil (medication vehicle). Testicular toxicity was induced in rats of the second and third groups by a single injection of diazinon (20 mg/kg, i.p.). Also, the animals of the second and third groups received the vehicle of TQ (olive oil) or TQ (10 mg/kg/day, i.p.), respectively, for five consecutive days starting 3 days before diazinon administration. The fourth group of animals received thymoquinone (10 mg/kg/day) for five consecutive days without induction of diazinon toxicity. Thirty-six hours later, semen quality was assessed for motility, morphology, and viability of sperms in addition to measurement of malondialdehyde (MDA) level in testis tissue. Results: The results showed that the sperm motility significantly decreased and MDA level significantly increased (P |
| Databáze: | Directory of Open Access Journals |
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