| Autor: |
Xia Feng, Jun-Wu Ma, Shi-Qi Sun, Hui-Chen Guo, Ya-Min Yang, Ye Jin, Guang-Qing Zhou, Ji-Jun He, Jian-Hong Guo, Shu-yun Qi, Mi Lin, Hu Cai, Xiang-Tao Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
PLoS ONE, Vol 11, Iss 3, p e0149569 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0149569 |
| Popis: |
The efficacy of an inactivated foot-and-mouth disease (FMD) vaccine is mainly dependent on the integrity of the foot-and-mouth disease virus (FMDV) particles. At present, the standard method to quantify the active component, the 146S antigen, of FMD vaccines is sucrose density gradient (SDG) analysis. However, this method is highly operator dependent and difficult to automate. In contrast, the enzyme-linked immunosorbent assay (ELISA) is a time-saving technique that provides greater simplicity and sensitivity. To establish a valid method to detect and quantify the 146S antigen of a serotype O FMD vaccine, a double-antibody sandwich (DAS) ELISA was compared with an SDG analysis. The DAS ELISA was highly correlated with the SDG method (R2 = 0.9215, P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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