| Autor: |
Konstantia Tsioupinaki, Stavros Spiliopoulos, Dimitris Karnabatidis, George Loudos, George C Nikiforidis, George C Kagadis |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Frontiers in Biomedical Technologies, Vol 1, Iss 1 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2345-5837 |
| Popis: |
Purpose: Integrin α β is a promising imaging target of angiogenic activity which is up-regulated on activated but not on quiescent endothelial cells. Molecular imaging of α β integrin expression with the aid of a dedicated high resolution gamma camera, is a very sensitive imaging approach for the evaluation of angiogenesis in the rabbit hindlimb ischemia model. Furthermore, in order to evaluate the whole spectrum of endogenous process of collateralization after occlusion of an artery, Digital Subtraction Angiography (DSA) was also used for the visualization of larger collaterals. Methods: The study included seven New Zealand White rabbits that underwent unilateral percutaneous endovascular embolization of the femoral artery, for the establishment of hindlimb ischemia that triggers the endogenous process of collateralization. The contralateral limb was not embolized and served as a control. The radiotracer that was employed for the angiogenesis imaging, was a 99 mTc labeled cyclic RGD peptide ([c RGDfk-His]-99mTc) that binds specifically to α β integrin via a three amino acid sequence (Arginine-Glycine-Aspartic acid or RGD). Image acquisition was performed with a high resolution gamma camera and all animals underwent molecular imaging on the 3rd day and the 9th day post-embolization. In all animals DSA was performed on the 9th day post-embolization. Results: The acquired images demonstrated the retention of the radiotracer at the ischemic tissue is remarkably increased compared to the non-ischemic hindlimb (normal limb) (mean value 16020 ± 2309 vs. 13139 ± 2493 on day 3; p=0.0014 and 21616 ± 2528 vs. 13362 ± 2529 on day 9; p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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