Bone marrow mesenchymal cells: polymorphism associated with transformation of rough endoplasmic reticulum
| Autor: | Yong-Xin Ru, Shu-Xu Dong, Chun-Hui Xu, Shi-Xuan Zhao, Hua-Mei Zhang, Hao-Yue Liang, Min Fen, Feng-Kui Zhang, Ying-Dai Gao, Shu-Lin Qi, Hong-Cai Shang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Blood Science, Vol 3, Iss 1, Pp 6-13 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2543-6368 00000000 |
| DOI: | 10.1097/BS9.0000000000000062 |
| Popis: | Abstract. To understand the behavior and function of bone-marrow mesenchymal cells (BMMCs), we overviewed the morphological presentation of BMMCs in bone-marrow granules (b-BMMCs), isolated BMMCs (i-BMMCs), and BMMCs (c-BMMCs) cultured in H4434 methylcellulose semisolid and MEM media. All samples were derived from bone-marrow aspirates of 30 patients with hematocytopenia. Light microscopy exhibited b-BMMCs and i-BMMCs characterized by abundant cytoplasm and irregular shape in bone-marrow smears, as well as c-BMMCs in culture conditions. Scanning electron microscopy demonstrated cultured c-BMMCs with a sheet-like feature enveloping hematopoietic cells. Transmission electron microscopy revealed b-BMMCs constructing a honeycomb-like structure by thin bifurcate processes among hematopoietic cells. Furthermore, i-BMMCs had bifurcate parapodiums on the surface and prominent rough endoplasmic reticulum (rER) connected with the plasmalemma of the parapodiums. The detailed images suggested that rER may serve as a membrane resource for plasmalemmal expansion in BMMCs in bone marrow. |
| Databáze: | Directory of Open Access Journals |
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