Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático Peptide sequencing using mass spectrometry: a practical guide

Autor: Marcelo Delmar Cantú, Emanuel Carrilho, Nelson Arno Wulff, Mario Sérgio Palma
Jazyk: English<br />Spanish; Castilian<br />Portuguese
Rok vydání: 2008
Předmět:
Zdroj: Química Nova, Vol 31, Iss 3, Pp 669-675 (2008)
Druh dokumentu: article
ISSN: 0100-4042
1678-7064
DOI: 10.1590/S0100-40422008000300034
Popis: This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.
Databáze: Directory of Open Access Journals