Estimation of Levetiracetam in Tablet Dosage Form by RP-HPLC
| Autor: | N. Appala Raju, J. Venkateswara Rao, K. Vanitha Prakash, K. Mukkanti, K. Srinivasu |
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| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: | |
| Zdroj: | E-Journal of Chemistry, Vol 5, Iss S2, Pp 1098-1102 (2008) |
| Druh dokumentu: | article |
| ISSN: | 0973-4945 2090-9810 |
| DOI: | 10.1155/2008/965430 |
| Popis: | A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of levetiracetam in tablet dosage form. A Sun Fire C18, 250 x 4.6 mm, 5 μm partical size, with mobile phase consisting of acetonitrile and 0.03 M potassium dihydrogen phosphate (pH adjusted to 3.0 with orthophosphoric acid) in the ratio of 15:85 v/v was used. The flow rate was 1 mL /min and the effluents were monitored at 210 nm. The retention time was 5.53 min. The detector response was linear in the concentration of 20-240 μg/mL. The respective linear regression equation being Y= 22119.684 x 6829.3428. The limit of detection and limit of quantification was 0.16 and 0.5 μg/mL respectively. The percentage assay of levetiracetam was 99.87%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of levetiracetam in bulk drug and in its pharmaceutical dosage form. |
| Databáze: | Directory of Open Access Journals |
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