Effect of equex on ram semen in different freezing extenders

Autor: Ebru ŞENGÜL, Cemal DAYANIKLI, Bilge ALPCAN KÜNYELİ, İsmail ÇOBAN, Bülent BÜLBÜL, Burcu ÜSTÜNER, Zekariya NUR
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 30, Iss 2, Pp 207-214 (2024)
Druh dokumentu: article
ISSN: 1309-2251
DOI: 10.9775/kvfd.2023.30789
Popis: This study was carried out to determine the effects of Equex on ram semen freezability in three different extenders. Semen samples were collected from five rams and divided into six equal aliquots. At first, three non-commercial base extenders (E1: Tris-based extender, E2: Tris based extender+ Cystein and E3: Skim Milk Powder (SMP)) were prepared. Each base extender was divided into two parts; a base extender (E1, E2 and E3) or fortified with Equex (E1E, E2E and E3E). The pooled semen was diluted according to the two-step dilution method, and cryopreserved using a programmable freezing machine. Sperm motility parameters were examined by CASA. The morphological properties were evaluated by flow cytometer. It was observed that the addition of Equex had a positive effect on post-thaw motility and live spermatozoa (P
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