Laser Phototherapy Enhances Mesenchymal Stem Cells Survival in Response to the Dental Adhesives

Autor: Ivana Márcia Alves Diniz, Adriana Bona Matos, Márcia Martins Marques
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Zdroj: The Scientific World Journal, Vol 2015 (2015)
Druh dokumentu: article
ISSN: 2356-6140
1537-744X
DOI: 10.1155/2015/671789
Popis: Background. We investigated the influence of laser phototherapy (LPT) on the survival of human mesenchymal stem cells (MSCs) submitted to substances leached from dental adhesives. Method. MSCs were isolated and characterized. Oral mucosa fibroblasts and osteoblast-like cells were used as comparative controls. Cultured medium conditioned with two adhesive systems was applied to the cultures. Cell monolayers were exposed or not to LPT. Laser irradiations were performed using a red laser (GaAlAs, 780 nm, 0.04 cm2, 40 mW, 1 W/cm2, 0.4 J, 10 seconds, 1 point, 10 J/cm2). After 24 h, cell viability was assessed by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide reduction assay. Data were statistically compared by ANOVA followed by Tukey’s test (P
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