Characterising side chains in large proteins by protonless 13C-detected NMR spectroscopy
Autor: | Ruth B. Pritchard, D. Flemming Hansen |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-7 (2019) |
Druh dokumentu: | article |
ISSN: | 2041-1723 |
DOI: | 10.1038/s41467-019-09743-4 |
Popis: | Analysis of side-chain motions by NMR has so far been restricted to small proteins and methyl-bearing side chains. Here, the authors present NMR methods based on 13C direct detection of highly deuterated protein samples that yield sharp and well-resolved signals and allow the characterisation of side-chain conformational dynamics of six different amino acid types in medium-to-large proteins. |
Databáze: | Directory of Open Access Journals |
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