Purification and Characterization β - lactamase produce from local isolate Klebsiella pneumonia
| Autor: | Baghdad Science Journal |
|---|---|
| Jazyk: | Arabic<br />English |
| Rok vydání: | 2009 |
| Předmět: |
characterization Enzyme get filtration storage activity. Science |
| Zdroj: | Baghdad Science Journal, Vol 6, Iss 1 (2009) |
| Druh dokumentu: | article |
| ISSN: | 2078-8665 2411-7986 |
| DOI: | 10.21123/bsj.6.1.50-60 |
| Popis: | Beta-lactamase was purified from local isolate Klebsiella pneumonia by several steps included precipitation with ammonium sulphate at 20-40% saturation, DEAE- ion exchange chromatography and gel filtration on Sephacryl S-200 column. The obtained purification fold and recovery were 32.66; 47.04% respectively. The characterization of the purified beta-lactamase showed that the molecular weight was about 4000 daltons as determined by gel filtration.Purified enzyme had an optimal pH of 7 for activity and an optimal stability between pH 6.5-7.5, results shows that the optimal temperature appear to be 35 ? C .During storage the enzyme retained 72% at -20 ? C and retained 25% of the activity at the same period at 4 ? C. |
| Databáze: | Directory of Open Access Journals |
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