| Autor: |
Efterpi Korakaki, Yannis Vasileios Simos, Niki Karouta, Konstantinos Spyrou, Panagiota Zygouri, Dimitrios Panagiotis Gournis, Konstantinos Ioannis Tsamis, Haralambos Stamatis, Evangelia Dounousi, Patra Vezyraki, Dimitrios Peschos |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Functional Biomaterials, Vol 14, Iss 10, p 514 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2079-4983 |
| DOI: |
10.3390/jfb14100514 |
| Popis: |
Superparamagnetic iron oxide nanoparticles (SPIONs) have garnered significant attention in the medical sector due to their exceptional superparamagnetic properties and reliable tracking capabilities. In this study, we investigated the immunotoxicity of SPIONs with a modified surface to enhance hydrophilicity and prevent aggregate formation. The synthesized SPIONs exhibited a remarkably small size (~4 nm) and underwent surface modification using a novel “haircut” reaction strategy. Experiments were conducted in vitro using a human monocytic cell line (THP-1). SPIONs induced dose-dependent toxicity to THP-1 cells, potentially by generating ROS and initiating the apoptotic pathway in the cells. Concentrations up to 10 μg/mL did not affect the expression of Nrf2, HO-1, NF-κB, or TLR-4 proteins. The results of the present study demonstrated that highly hydrophilic SPIONs were highly toxic to immune cells; however, they did not activate pathways of inflammation and immune response. Further investigation into the mechanisms of cytotoxicity is warranted to develop a synthetic approach for producing effective, highly hydrophilic SPIONs with little to no side effects. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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