Engineered cyanobacteria-Fe3O4 hybrid system as oxygen generator and photosensitizer production factory for synergistic cancer PDT-immunotherapy

Autor: Yize Li, Yali Fan, Jin Gao, Shaohui Zheng, Yujuan Xing, Chunyan He, Shuo Ye, Hongfei Xia, Gezhen Wang, Hui Pan, Wei Xia, Meirong Sui, Hanjie Wang, Jing Liu, Manman Xie, Kai Xu, Yingying Zhang
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Materials Today Bio, Vol 28, Iss , Pp 101192- (2024)
Druh dokumentu: article
ISSN: 2590-0064
DOI: 10.1016/j.mtbio.2024.101192
Popis: The combination of photodynamic therapy (PDT)-immunotherapy has brought much hope for cancer patients. However, the hypoxia tumor microenvironment (TME) can regulate tumor angiogenesis and inhibit immune response, thus limiting the therapeutic effects. In this paper, engineered cyanobacteria-M2-like tumor-associated macrophages (TAMs) targeting peptide modified Fe3O4 nanoparticles hybrid system (ECyano@Fe3O4-M2pep) was constructed for alleviating hypoxia and relieving immune suppression to achieve synergistic cancer PDT-immunotherapy. With the irradiation of red laser, oxygen was produced by the photosynthesis of ECyano to alleviate the hypoxia TME. Then, ECyano could secret 5-aminolevulinic acid (5-ALA) under the induction of theophylline for controllable PDT. In the process of PDT, the disulfide bond between ECyano and Fe3O4-M2pep was broken in response to reactive oxygen species (ROS), and then Fe3O4-M2pep was released to target M2-like TAMs, corresponding by the polarization of M2-like TAMs to M1-like TAMs for the killing of tumor cells. Compared with other groups, ECyano@Fe3O4-M2pep + theophylline + laser (ECyano@Fe3O4-M2pep + T + L) group displayed the lowest tumor volume (159.3 mm3) and the highest M1/M2 ratio (1.25- fold). We believe that this hybrid system will offer a promising way for the biomedical application of bacterial therapy.
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