Towards an Ideal In Cell Hybridization-Based Strategy to Discover Protein Interactomes of Selected RNA Molecules

Autor:	Michele Spiniello, Mark Scalf, Amelia Casamassimi, Ciro Abbondanza, Lloyd M. Smith
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	RNA molecules ‘in cell’ hybridization methods RNA–protein interactions Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 23, Iss 2, p 942 (2022)
Druh dokumentu:	article
ISSN:	1422-0067 1661-6596
DOI:	10.3390/ijms23020942
Popis:	RNA-binding proteins are crucial to the function of coding and non-coding RNAs. The disruption of RNA–protein interactions is involved in many different pathological states. Several computational and experimental strategies have been developed to identify protein binders of selected RNA molecules. Amongst these, ‘in cell’ hybridization methods represent the gold standard in the field because they are designed to reveal the proteins bound to specific RNAs in a cellular context. Here, we compare the technical features of different ‘in cell’ hybridization approaches with a focus on their advantages, limitations, and current and potential future applications.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/e0d0fdc49ffe4f8dbbee0d4fe0383f0a Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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