Antimicrobial susceptibility testing by broth microdilution method: widely available modification
| Autor: | Tapalskiy D.V., Bilskiy I.A. |
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| Jazyk: | ruština |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Клиническая микробиология и антимикробная химиотерапия, Vol 20, Iss 1, Pp 62-67 (2018) |
| Druh dokumentu: | article |
| ISSN: | 1684-4386 2686-9586 |
| Popis: | Objective. To assess the possibility of standard disk application as an antibiotic source for determination of the minimal inhibitory concentrations (MICs) by serial microdilution method. Materials and Methods. A simple method of working solutions preparation using standard paper discs as an antibiotics source available in most microbiological laboratories is proposed. A multiple determination of antibiotic MICs for E. coli ATCC 25922 and P. aeruginosa ATCC 27853 strains was conducted. Results. Of the 576 MIC values determined by the modified method, 78.5% of the results corresponded to the control strains reference values, and 20.8% of the results were categorized as acceptable values. Conclusions. Use of standard disks as an antibiotics source for the working solutions preparation greatly simplifies a testing by serial microdilution method and makes it available to most microbiological laboratories. |
| Databáze: | Directory of Open Access Journals |
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