Contactless crystallization method of protein by a magnetic force booster

Autor: Syou Maki, Masayuki Hagiwara
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)
Druh dokumentu: article
ISSN: 2045-2322
DOI: 10.1038/s41598-022-21727-x
Popis: Abstract We developed a new type of compact magnetic force booster by which we succeeded in crystallizing proteins (hen egg white lysozyme) while making them levitate in a solution without contacting the container. This technique is noteworthy in the practical merit that we could control the growth of crystals from the initial stage of nucleation in a magnetic field of merely a few Tesla. The shape of the booster was designed in accordance with the dynamical stability against external forces acting on the crystals. Under a stable condition, the crystals condensed spherically, and formed a “shell shaped” crystallization with a hollow interior. Our magnetic force booster has the potential for use in innovation, especially in the field of protein crystal engineering.
Databáze: Directory of Open Access Journals
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