| Autor: |
Sheng-Yu Huang, Chih-Heng Huang, Chi-Jene Chen, Ting-Wen Chen, Chun-Yuan Lin, Yueh-Te Lin, Shu-Ming Kuo, Chung-Guei Huang, Li-Ang Lee, Yi-Hsiang Chen, Mei-Feng Chen, Rei-Lin Kuo, Shin-Ru Shih |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Molecular Therapy: Nucleic Acids, Vol 17, Iss , Pp 10-23 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2162-2531 |
| DOI: |
10.1016/j.omtn.2019.04.028 |
| Popis: |
The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3′ UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication. Keywords: influenza A virus, miR-1290, vimentin, viral ribonucleoprotein, vRNP, miRNA, virus, host species-specificity, ferret |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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