| Autor: |
Hidekazu Nakai, MD, Yasuyuki Fujita, MD, PhD, Satoru Masuda, MT, Miki Komatsu, MT, Ayumi Tani, MT, Yutaka Okita, MD, PhD, Kenji Okada, MD, PhD, Atsuhiko Kawamoto, MD, PhD |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
JTCVS Open, Vol 7, Iss , Pp 23-40 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2666-2736 |
| DOI: |
10.1016/j.xjon.2021.06.008 |
| Popis: |
Objective: We sought to investigate the efficacy of human bone marrow mesenchymal stem/stromal cell (hBM-MSC) in a murine spinal cord ischemia/reperfusion (SCIR) model. Methods: C57BL/6J mice were subjected to SCIR by crossclamping the aortic arch and left subclavian artery for 5.5 minutes. Two hours after reperfusion, hBM-MSCs (hBM-MSC group) or phosphate-buffered saline (control group) were intravenously injected without immunosuppressant. Hindlimb motor function was assessed until day 28 after reperfusion using the Basso Mouse Scale (BMS). The lumbar spinal cord was harvested at hour 24 and day 28, and the histologic number of NeuN-positive motor neurons in 3 cross-sections of each lumbar spinal cord and the gene expression were evaluated. Results: BMS score was 0 throughout the study period in all control mice. BMS score was significantly greater in the hBM-MSC group than the control group from hour 8 (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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