Identification and Fine Mapping of Quantitative Trait Loci for Tiller Angle Using Chromosome Segment Substitution Lines in Rice (Oryza Sativa L.)

Autor: Yujia Leng, Tao Tao, Shuai Lu, Ran Liu, Qingqing Yang, Mingqiu Zhang, Lianmin Hong, Qianqian Guo, Xinzhe Ren, Zhidi Yang, Xiuling Cai, Sukui Jin, Jiping Gao
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Agriculture, Vol 14, Iss 7, p 1002 (2024)
Druh dokumentu: article
ISSN: 14071002
2077-0472
DOI: 10.3390/agriculture14071002
Popis: The tiller angle, which is an important agronomic trait, determines plant architecture and greatly influences the grain yield of rice. In this study, a population of chromosome segment substitution lines derived from a cross between a japonica variety with a compact plant architecture—Koshihikari—and an indica variety with a spread-out plant architecture—Nona Bokra—was used to investigate the genetic basis of the tiller angle. Five quantitative trait loci (qTA1, qTA5, qTA9-1, qTA9-2, and qTA11) for the tiller angle were detected on chromosomes 1, 5, 9, 9, and 11 in two different environments. The phenotypic variation in these QTLs ranged from 3.78% to 8.22%. Two pairs of digenic epistatic QTLs were detected in Lingshui. The epistatic interaction explained 15.19% and 13.60% of the phenotypic variance, respectively. Among the five QTLs, qTA9-2 was detected in both environments. An F2 mapping population containing the qTA9-2 QTL was established. The location of qTA9-2 was narrowed down to a 187 kb region between InDel markers M9 and M10 on chromosome 9. Thirty open reading frames (ORFs), including TAC1, a gene known to regulate the tiller angle, were identified in this region. The gene sequencing results suggested that a base substitution from G to A at position 1557 in the 3′-untranslated region led to a difference in the expression of qTA9-2 in Koshihikari and Nona Bokra. These findings provide a potential gene resource for the improvement of rice plant architecture.
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