Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model
| Autor: | Po’okela K. Ng, BS, Dor Yoeli, MD, Joy L. Huang, BA, Yuhuan Luo, MD, Yong Wang, MD, Bing Li, MD, Zhaohui Wang, DVM, Jesse Schold, PhD, Swati Jain, PhD, An-Jey A. Su, PhD, David W. Mathes, MD, Kia M. Washington, MD, Evan Farkash, MD, PhD, Alkesh H. Jani, MD, Christene A. Huang, PhD |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Transplantation Direct, Vol 10, Iss 6, p e1623 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2373-8731 00000000 |
| DOI: | 10.1097/TXD.0000000000001623 |
| Popis: | Background. Vascularized composite allograft transplantation is a treatment option for complex tissue injuries; however, ischemia reperfusion injury and high acute rejection rates remain a challenge. Hypothermic machine perfusion using acellular storage perfusate is a potential solution. This study evaluated the University of Wisconsin Kidney Preservation Solution-1 (KPS-1) compared with normal saline (NS) for preservation of donor rat hindlimbs subjected to 24 h of ex vivo perfusion cold storage. Methods. Hindlimbs were subjected to 24-h perfusion cold storage with heparinized KPS-1 (n = 6) or heparinized NS (n = 6). Flow, resistance, and pH were measured continuously. At the end of the 24-h period, tissue was collected for histological analysis of edema and apoptosis. Results. KPS-1 perfused limbs showed significantly less edema than the NS group, as evidenced by lower limb weight gain (P |
| Databáze: | Directory of Open Access Journals |
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