Comparison of cytotoxic effects of calcium silicate-based materials on human pulp fibroblasts
Autor: | Mehmet Adıgüzel, Fuat Ahmetoğlu, Ayçe Ünverdi Eldeniz, Mehmet Gökhan Tekin, Bülent Göğebakan |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | Journal of Dental Research, Dental Clinics, Dental Prospects, Vol 13, Iss 4, Pp 241-246 (2019) |
Druh dokumentu: | article |
ISSN: | 2008-210X 2008-2118 |
DOI: | 10.15171/joddd.2019.037 |
Popis: | Background. This study aimed to compare the in vitro cytotoxicity of Theracal LC, BiodentineTM, iRoot BP Plus, and MTA Angelus on human pulp fibroblasts (HPF). Methods. Fifteen discs from each calcium silicate-based material were prepared in sterile Teflon molds. After setting, the fabricated discs were eluated with a culture medium for 24 h. HPF cells were plated onto 24-well plates at 5×103 cells/well, and the cells were exposed to the material eluates. The cell viability was evaluated with MTT assay at three different times (24, 48, and 72 h). Data were statistically analyzed. The apoptotic/necrotic status of HPF cells exposed to material eluates was determined by flow cytometry. Results. The differences between the effects of Theracal LC, BiodentineTM, MTA Angelus, and iRoot BP Plus on HPF cells were found to be statistically significant (P |
Databáze: | Directory of Open Access Journals |
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