| Autor: |
Avrin Ghanaeian, Sumita Majhi, Caitlyn L. McCafferty, Babak Nami, Corbin S. Black, Shun Kai Yang, Thibault Legal, Ophelia Papoulas, Martyna Janowska, Melissa Valente-Paterno, Edward M. Marcotte, Dorota Wloga, Khanh Huy Bui |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-023-41480-7 |
| Popis: |
Abstract Cilia are hairlike protrusions that project from the surface of eukaryotic cells and play key roles in cell signaling and motility. Ciliary motility is regulated by the conserved nexin-dynein regulatory complex (N-DRC), which links adjacent doublet microtubules and regulates and coordinates the activity of outer doublet complexes. Despite its critical role in cilia motility, the assembly and molecular basis of the regulatory mechanism are poorly understood. Here, using cryo-electron microscopy in conjunction with biochemical cross-linking and integrative modeling, we localize 12 DRC subunits in the N-DRC structure of Tetrahymena thermophila. We also find that the CCDC96/113 complex is in close contact with the DRC9/10 in the linker region. In addition, we reveal that the N-DRC is associated with a network of coiled-coil proteins that most likely mediates N-DRC regulatory activity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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