Autor: |
Aanchal Yadav, Priti Devi, Pallawi Kumari, Ranjeet Maurya, Uzma Shamim, Rajesh Pandey |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
|
Zdroj: |
STAR Protocols, Vol 5, Iss 2, Pp 103071- (2024) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2024.103071 |
Popis: |
Summary: The elucidation of the role of microorganisms in human infections has been hindered by difficulties using conventional culture-based techniques. Here, we present a protocol for the investigation of transcriptionally active microbes (TAMs) using an RNA sequencing (RNA-seq)-based approach. We describe the steps for RNA isolation, viral genome sequencing, RNA-seq library preparation, and metatranscriptomic and transcriptomic analysis. This protocol permits a comprehensive evaluation of TAMs’ contributions to the differential severity of infectious diseases, with a particular focus on diseases such as COVID-19.For complete details on the use and execution of this protocol, please refer to Devi et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
|