Autor: |
Liangkang Lv, Fengliang Xiong, Yingyi Liu, Shiteng Pei, Shanshan He, Shengli Li, Hongjian Yang |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
BMC Microbiology, Vol 24, Iss 1, Pp 1-14 (2024) |
Druh dokumentu: |
article |
ISSN: |
1471-2180 |
DOI: |
10.1186/s12866-023-03156-6 |
Popis: |
Abstract Background This study aimed to isolate the rumen-derived bacteria with the ability to degrade free gossypol (FG), and to evaluate the probiotic potential in vitro for ensuring safe utilization. Methods The strains were anaerobically isolated from fresh rumen fluid of sheep with long-term fed cottonseed meal (CSM) with the screening agar medium containing gossypol as the sole carbon source. Afterwards, the isolated strain incubated with CSM was subjected to the determination of the FG degradation and in vitro evaluation of probiotic characteristics. Results The target strain labeled Lact. mucosae LLK-XR1 [Accession number: OQ652016.1] was obtained, and its growth on MRS Liquid medium exhibited degradation efficiency of FG up to 69.5% which was significantly greater than its growth on Man-Rogosa-Sharpe medium with glucose free for 24 h (p |
Databáze: |
Directory of Open Access Journals |
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