Comparative Evaluation of Serological, ELISA and Molecular Tests in Diagnosis of Brucella abortus in Human Serum
| Autor: | Fereshteh Ghanbari, Haniyeh Bashi Zadeh Fakhar, Saeed Alamian, Babak Shaghaghi |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Journal of Medical Bacteriology, Vol 11, Iss 5-6 (2023) |
| Druh dokumentu: | article |
| ISSN: | 2251-8649 2322-2581 |
| DOI: | 10.18502/jmb.v11i5-6.14361 |
| Popis: | Background: Brucellosis is one of the most prevalent and common diseases between humans and animals.Prompt diagnosis and timely treatment of this disease can prevent many complications. In this regard, this study aims to comparatively evaluate ELISA, PCR and serological methods to identify Brucella abortus. Methods: In this study, the serum of 100 patients referred to Tonekabon private laboratory from July 2020 to January 2021 was examined by PCR, ELISA and Wright, Coombs Wright, and 2ME methods for the detection of Brucella abortus. Results: In this study, the mean age of the sample was 43.3 ± 18.2 of which 21% were infected with Brucella abortus according to the above serological methods. According to ELISA test, 22% of the samples were IgM, 6% of the samples were IgG and 16% were PCR positive. Kappa agreement coefficient in Wright and Coombs Wright test and 2me were significant (P |
| Databáze: | Directory of Open Access Journals |
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