EFFECT OF ANTIBIOTICOTHERAPY ON THE TOTAL AMOUNT OF OXALATE-DEGREADING BACTERIA IN THE RATS INTESTINAL TRACT
| Autor: | І. В. Акуленко, М. Ю. Корбуш, В. О. Стецька, Т. М. Сергійчук, Г. М. Толстанова, Н. М. Степанова |
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| Jazyk: | English<br />Russian<br />Ukrainian |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Mìkrobìologìâ ì Bìotehnologìâ, Vol 0, Iss 2(46), Pp 27-37 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2076-0558 2307-4663 |
| DOI: | 10.18524/2307-4663.2019.2(46).159163 |
| Popis: | Aim. To determine the quantity of oxalate-degrading bacteria (ODB) in fecal biopsy and mucosa-associated microbiota of the colon and small intestine of rats. Methods. Study was conducted on male Wistar rats (weight 170–200 g, n=7). The object of the study was the change in the total amount of oxalate-degrading microbiota of the fecal and mucosa-associated biotope of the colon and small intestine. The mix of ampicillin (75 mg/kg) and metronidazole (50 mg/kg) was injected once a day, for 3 days per os. The dynamics of microbiota changes were evaluated immediately after the antibiotics withdrawals, on the 18th, 29th and 59th days of the experiment. The microbiological analysis of fecal (CFU/g) and mucosa-associated (CFU/cm2 ) biotope of rats was carried out bacteriologically by sowing the according dilutions on elective media. The results are presented in the form of M±m lg CFU/g (cm2 ). Results. The number of bacteria from the fecal biopsy sowed in a highly selective Oxalate Medium (5 g/l Na2 C2 O4 ) decreased from 6,22±0,20 to 4,37±0,80 lg CFU/g (Р |
| Databáze: | Directory of Open Access Journals |
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