| Autor: |
Blair Thomson, Jess Wenley, Scott Lockwood, Isla Twigg, Kim Currie, Gerhard J. Herndl, Christopher D. Hepburn, Federico Baltar |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Frontiers in Earth Science, Vol 8 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2296-6463 |
| DOI: |
10.3389/feart.2020.560893 |
| Popis: |
Marine microbes use extracellular phosphatases to hydrolyze phosphate from organic matter. Dissolved organic phosphorus (DOP) is typically present in higher concentrations than phosphate in oceanic surface waters. Yet, the fate and role of different DOP components, such as phosphomonoester and phosphodiester, are poorly understood. Most of the investigations on extracellular enzymatic hydrolysis of marine DOP have focused on phosphomonoesterase (MEA) activity (i.e., alkaline phosphatase), whereas phosphodiesterase (DEA) measurements are scarce. This limits our understanding of the ecological and biogeochemical role of DOP sources other than P-monoesters in the sea. We determined extracellular MEA and DEA activities including their cell-free fractions on a bimonthly basis over 14 months in surface and mesopelagic subantarctic waters, thus covering a wide range of phosphate availability levels (from 70%) of extracellular MEA and DEA was found in the cell-free fraction, increasing with depth for MEA. Our results indicated that DOP hydrolysis mediated by DEA in the surface as well as in dark ocean is as important as the frequently measured MEA. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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