The protective effect of vitamin E and selenium on liver tissue in fructose- induced diabetic rat
| Autor: | Farangis Ghassemi, amin Yuosefinasab |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | فصلنامه دانشگاه علوم پزشکی جهرم, Vol 12, Iss 2, Pp 47-55 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2008-7993 2008-8442 |
| Popis: | Background: Diabetes disorder in organ's function via oxidative stress. In this research, the protective effect of vitamin E and selenium "as antioxidant" on liver function were investigeted in diabetic rats. Materials and methods: 35 adult male rats (Wistar) weighing 180 ± 20g divided randomly into control, sham and 3 experimental groups (E, S and E+S). All of groups except control were fed 140 cc fructose 10% as a daily for 9 weeks. Control group without treatment, sham were injected 0.20 cc of physiologic serum and and group (S) were injected 0.20 cc of selenium solution (0.5 mg/kg/b.wt) intraperitoneally. group (E) were gavaged 0.20 cc of vitamin E solution (0.5 mg/kg/b.wt) and Group (E + S) gavaged vitamin E and were injected selenium (0.5mg/kg/b.wt) for 9 weeks. Blood sampling of all groups and were separated for biochemical analyzes. Then liver was disected and fixed in 3% formalin. hbafty Automatic Tissue processor with 5 micrometer and were examined by light microscope. The sections with 5 micron thickness were prepared by microtome after passage in Tissue processor automatically. They were studied on light microscope. Using statistical software, SPSS 17, data were analyzed by ANOVA and groups were compared by Touki test at a significance level (P |
| Databáze: | Directory of Open Access Journals |
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