| Autor: |
Jamie Richards, Joel G. Belasco |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 2, Pp 101190- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101190 |
| Popis: |
Summary: Generated by RNA deprotection or cleavage, 5′ monophosphates trigger RNA degradation in all organisms. Here we describe PABLO-QA (Phosphorylation Assay By Ligation of Oligonucleotides and Quantitative Amplification), a sensitive, low-cost procedure for determining the percentage of specific RNA 5′ ends that are monophosphorylated from their ability to undergo ligation to an oligonucleotide. Comparison to a cognate internal standard and a fully monophosphorylated control allows precise quantification of monophosphorylated 5′ termini by RT-PCR, enabling the analysis of transcripts undetectable by blotting.For complete details on the use and execution of this protocol, please refer to Richards and Belasco (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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