| Autor: |
Jia XU, Kunpeng FENG, Zhiqiang ZHANG, Yingsai WANG |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Hebei University of Science and Technology, Vol 44, Iss 5, Pp 534-540 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1008-1542 |
| DOI: |
10.7535/hbkd.2023yx05012 |
| Popis: |
In order to reduce the production cost of γ-polyglutamic acid (γ-PGA) and realize high-value utilization of renewable resources such as reed, the feasibility of establishing a new fermentation process control strategy for the preparation of γ-PGA using reed hydrolysate as carbon source was explored. Firstly, the effects of cellulase dosage and substrate concentration on reed hydrolysis were studied; Secondly, the process of fermentation and production of γ-PGA from reed hydrolysate was optimized by segmented culture and DO feed-back control strategies. The results show that after pretreatment, the total sugar concentration of reed could reach (7664±174)g/L under the condition of 10 FPU/g (substrate) enzyme dosage and 10% substrate addition; the reed hydrolysate was produced γ-PGA by segmented dissolved oxygen feedback-batch feed fermentation culture, and the final yield could reach (4672±118])]g/L, with the production efficiency increasing by 4606% compared with batch fermentation. The use of reed hydrolysate for batch fermentation of γ-PGA is industrially feasible, which helps to reduce the raw material cost of its commercial production. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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