INFLUENCE OF POLYMYXIN B ON THE FORMATION OF BIOFILMS BY BACTERIUM Methylophilus quaylei ON POLYPROPYLENE AND TEFLON
| Autor: | A. M. Mohamed, D. N. Amzaeva, A. B. Pshenichnikova, V. I. Shvets |
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| Jazyk: | ruština |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Тонкие химические технологии, Vol 13, Iss 2, Pp 31-39 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2410-6593 2686-7575 |
| DOI: | 10.32362/2410-6593-2018-13-2-31-39 |
| Popis: | Here we examined the influence of polymyxin B, a lipopeptide antibiotic, on planktonic growth and biofilm of streptomycin-sensitive and streptomycin-resistant isogenic strains of bacteria Methylophilus quaylei on polypropylene and Teflon. Biofilm formation was quantified using crystal violet staining, determined by colony-forming unit and examined by light microscopy. It was found that of bacterial surface hydrophobicity affects the biofilm formation: biofilm formation of Methylophilus quaylei MT strain was better on such hydrophobic materials as Teflon and polypropylene. The minimum inhibitory concentration of polymyxin B for the used strains in planktonic and biofilm form was 1 μg/ml. The sublethal Polymyxin B in the concentration of 0.01 μg/ml stimulates biofilm formation and exhibits antibiofilm properties at the concentration of 0.10 μg/ml. Synergistic effect of polymyxin B and streptomycin on streptomycin-resistant strain M. quaylei SM was found. |
| Databáze: | Directory of Open Access Journals |
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