| Autor: |
V. Goranova, M. Sifringer, C. Ikonomidou |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Trakia Journal of Sciences, Vol 17, Iss Suppl. 2, Pp 113-122 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1313-3551 |
| DOI: |
10.15547/tjs.2019.s.02.025 |
| Popis: |
PURPOSE. Mechanical trauma to the immature rodent brain induces a local primary injury and a distant secondary neuroapoptosis. These lesions are associated with glial cell activation, infiltration of immune cells, increased levels of proinflammatory mediators and proteases as part of neuroinflammation. METHODS. Seven-day-old rats were subjected to cortical trauma with a weight-drop device. Animals were sacrificed at defined time points from 2 hours to 21 days and brain tissues were processed for molecular, biochemical and histological analyses. RESULTS. We studied the expression, distribution pattern, time course and cellular localization of two pro-inflammatory mediators (IL-1ß and IL-18) and two proteases (MMP-2 and MMP-9). Apoptotic cell death affected mostly neuronal populations in the cortex, thalamus and caudate nucleus mainly ipsilateral to the injury 6 hours to 5 days after trauma. The involvement of microglia, astroglia and monocytesmacrophages were investigated at the site of primary and secondary damages. They may contribute to acute or delayed trauma-induced neuronal deficit. CONCLUSIONS. Our findings suggest that the interleukins, proteases and microglia/astrocytes are involved in trauma-induced neuroinflammatory response and might serve as a potential therapeutic target. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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