| Autor: |
Jaime Parra S, Jorge Agudelo T, David Sanín P, Jorge Forero D, Carlos Muskus L, Albeiro López Herrera |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
|
| Zdroj: |
Revista Colombiana de Ciencias Pecuarias, Vol 26, Iss 2, Pp 108-118 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
0120-0690 |
| Popis: |
Background: treatments with lipopolysaccharide (LPS) from E. coli are an accepted way of inducing inflammation in immunological studies since they have the ability to activate a coordinate series of signs through the synthesis of pro-inflammatory cytokines such as interleukin 8 (IL-8), IL-18 and tumor necrosis factor alpha (TNF-α), which can cause significant changes in intestinal structure and functionality. Objective: the aim of this study was to evaluate the effect of adding LPS of E. coli on pro-inflammatory cytokine gene expression IL-8, IL-18 and TNF-α in early-weaned pigs. Methods: fieldwork was conducted at Centro San Pablo, belonging to the Universidad Nacional de Colombia with 32 pigs at 21 days of age, with 6.5 ± 0.5 kg of weight. Animals were fed with a basal diet supplemented with two levels of inclusion of LPS of E. coli serotype 0111:B4 (0 to 0.3 μg/mg of food). Pigs were slaughtered in stages on days 1, 5, 7 and 10 postweaning and complete extraction of small intestine was made. Gene expression was evaluated by qPCR. Blocks at random in a factorial arrangement 2 x 4 were used as statistical design. Results: the basal diet (without addition of LPS) presented increase in mRNA expression (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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