The effects of ovarian encapsulation with alginate hydrogel on morphology and follicular count of vitrified mouse ovary
| Autor: | Shirazi-Tehrani A, Mazoochi T, Akhavan-Taheri M, Salehnia M |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Fiyz̤, Vol 23, Iss 5, Pp 467-475 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1029-7855 2008-9821 |
| Popis: | Background: Ovarian tissue cryopreservation is a way of preserving fertility in women with cancer and premature ovarian failure. Maintenance of follicular reserve is mandatory after ovary cryopreservation. This study was performed to determine the effect of alginate hydrogel as a capsule on morphology and follicular count of vitrified mouse ovary. Materials and methods: In this experimental study, mature mice ovaries were divided into four groups: non-vitrified ovary, vitrified ovary, ovaries that have been encapsulated with alginate hydrogel with a concentration of 0.5 and 1% and then were vitrified (Experimental1 and Experimental2, respectively). The vitrification was performed with ethylene glycol and dimethyl sulfoxide solutions. Morphological study was done using hematoxylin and eosin staining. Average number of intact and atretic follicles was evaluated in each group. Data were compared by one-way ANOVA and post hoc test. Values of P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|