| Autor: |
Dae-Jung Kim, Chae-Won Park, Munkhzaya Byambaragchaa, Shin-Kwon Kim, Bae-Ik Lee, Hyung-Kyu Hwang, Jeong-In Myeong, Sun-Mee Hong, Myung-Hwa Kang, Kwan-Sik Min |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Data in Brief, Vol 8, Iss , Pp 404-410 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3409 |
| DOI: |
10.1016/j.dib.2016.05.069 |
| Popis: |
Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography.In support of our recent publication, ''Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica'' [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary. Keywords: Japanese eel, FSH, Monoclonal Antibody |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
