The Reverse Side of a Coin: 'Factor-Free' Ribosomal Protein Synthesis In Vitro is a Consequence of the In Vivo Proofreading Mechanism

Autor:	Alexei V. Finkelstein
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	ribosome biosynthesis of polypeptides elongation factor tu ef-tu gtp factor-free process factor-promoted process parallel reactions free energy anticodon-codon recognition complementarity non-complementarity binding of aa-trna proofreading removal of aa-trna Microbiology QR1-502
Zdroj:	Biomolecules, Vol 9, Iss 10, p 588 (2019)
Druh dokumentu:	article
ISSN:	2218-273X
DOI:	10.3390/biom9100588
Popis:	This paper elucidates a close connection between two well-known facts that until now have seemed independent: (i) the quality control (“proofreading”) of the emerging amino acid sequence, occurring during the normal, elongation-factor-dependent ribosomal biosynthesis, which is performed by removing those Aa-tRNAs (aminoacyl tRNAs) whose anticodons are not complementary to the exhibited mRNA codons, and (ii) the in vitro discovered existence of the factor-free ribosomal synthesis of polypeptides. It is shown that a biological role of proofreading is played by a process that is exactly opposite to the step of factor-free binding of Aa-tRNA to the ribosome-exposed mRNA: a factor-free removal of that Aa-tRNA whose anticodon is not complementary to the ribosome-exhibited mRNA codon.
Databáze:	Directory of Open Access Journals
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