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Objective: To compare the positive rate and average time for slide reading of fluorescent staining versus KOH wet-mount microscopy in the diagnosis of different types of tinea pedis. Methods: Specimens were collected from 118 cases of blister scaly type tinea pedis, 104 cases of cornification excess type tinea pedis and 78 cases of soak erosion type tinea pedis. These specimens were subjected to fluorescent staining and KOH wet mount separately, followed by KOH wet-mount microscopy. The positive rate and average time for slide reading were compared between the two methods. Results: The background of fluorescent staining method was relatively clean, and the fungi was clearly identifiable, while the background of KOH wet-mount method was messy and it was difficult to identify fungi. In three different types of tinea pedis, the positive rates were 91.53%, 75.96% and 98.72%, respectively using fluorescent staining method, while the positive rates were 80.51%, 47.12% and 97.44%, respectively when KOH wet mount was used. The positive rates for blister scaly type tinea pedis and cornification excess type tinea pedis were significantly different between the two methods(χ2=5.33, 20.45 respectively, both P |
