Autor: |
Biola F. Egbowon, Enzo Fornari, Joseph M. Pally, Alan J. Hargreaves, Bob Stevens, T. Martin McGinnity, Barbara K. Pierscionek |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
|
Zdroj: |
Materials & Design, Vol 232, Iss , Pp 112152- (2023) |
Druh dokumentu: |
article |
ISSN: |
0264-1275 |
DOI: |
10.1016/j.matdes.2023.112152 |
Popis: |
Engineered tissue currently lacks requisite capacity to sustain cell viability and functionality. Here we demonstrate that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without (untreated) or with (treated) fluocinolone acetonide (FA). Cells survived and retained their characteristic morphology for up to 150 days with FA-treated ENS and manifested a morphological epithelial phenotype with expression of biomarkers critical for maintaining retinal physiological characteristics. This novel technique for producing culture substrates provides suitable hydrophilicity and a protective environment for prolonged RPE culture and has immense potential for subretinal transplantation. The findings indicate that FA-treated ENS is an excellent matrix for retaining the differentiated and epithelial phenotype. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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