Improved betulinic acid biosynthesis using synthetic yeast chromosome recombination and semi-automated rapid LC-MS screening

Autor: G.-O. F. Gowers, S. M. Chee, D. Bell, L. Suckling, M. Kern, D. Tew, D. W. McClymont, T. Ellis
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Nature Communications, Vol 11, Iss 1, Pp 1-7 (2020)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-020-14708-z
Popis: SCRaMbLE can lead to great genetic diversity for product biosynthesis but is limited by screening methods. Here the authors develop a rapid workflow using automation, ultra-fast LC/MS and barcoded nanopore sequencing to identify best performing strains.
Databáze: Directory of Open Access Journals