Virulence role of the outer membrane protein CarO in carbapenem-resistant Acinetobacter baumannii
| Autor: | Gema Labrador-Herrera, Antonio J. Pérez-Pulido, Rocío Álvarez-Marín, Carlos S. Casimiro-Soriguer, Tania Cebrero-Cangueiro, Jorgelina Morán-Barrio, Jerónimo Pachón, Alejandro M. Viale, María Eugenia Pachón-Ibáñez |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Virulence, Vol 11, Iss 1, Pp 1727-1737 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2150-5594 2150-5608 21505594 |
| DOI: | 10.1080/21505594.2020.1855912 |
| Popis: | Novel approaches to treat carbapenem-resistant Acinetobacter baumannii (CRAB) infections are urgently needed and anti-virulence drugs represent promising alternatives, but our knowledge on potential targets is scarce. We searched for potential A. baumannii virulence factors by whole-genome sequencing-based comparisons of CRAB clinical isolates causing bloodstream infections secondary to ventilator-associated pneumonia from demographics and clinically homogeneous patients, who received optimal treatment but with different clinical outcomes. Thus, the carO gene was interrupted in CRAB isolates from surviving patients, while it was intact in isolates from non-surviving patients, and proteomic/immunoblot techniques corroborated it. When the virulence role of A. baumannii CarO was analyzed in model systems, isogenic ΔcarO mutants and a CRAB clinical isolate with truncated CarO, showed lower ability to adhere and invade A549 cells and in vivo virulence. This unnoticed virulence role for CarO postulate this A. baumannii outer membrane protein as a potential target for new therapies against CRAB infections. |
| Databáze: | Directory of Open Access Journals |
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