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The study was aimed to determine the optimal dietary manganese (Mn) requirement by evaluating the effects of various dietary Mn levels on the growth performance, whole-body chemical composition, bone mineralization, and haematology of juvenile stinging catfish Heteropneustes fossilis. Five isonitrogenous purified diets containing graded levels of supplemental Mn from MnSO4.H2O (0, 5, 10, 15 and 20 mg Mn kg−1) were formulated, and referred to as Mn0, Mn5, Mn10, Mn15, and Mn20, respectively. Fish (mean weight of 1.24 g) were stocked in 15 glass aquaria (40 fish per aquarium) each of 180 L water capacity. Each diet was hand-fed twice a day (9.00 and 16.00 h) to apparent satiation to triplicate groups of fish, 7 days a week for 10 weeks. Results indicated that significantly (P |
