Autor: |
Dezerae Cox, Angelique R. Ormsby, Gavin E. Reid, Danny M. Hatters |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
npj Systems Biology and Applications, Vol 8, Iss 1, Pp 1-10 (2022) |
Druh dokumentu: |
article |
ISSN: |
2056-7189 |
DOI: |
10.1038/s41540-022-00256-3 |
Popis: |
Abstract The correct spatio-temporal organization of the proteome is essential for cellular homeostasis. However, a detailed mechanistic understanding of this organization and how it is altered in response to external stimuli in the intact cellular environment is as-yet unrealized. ‘Protein painting methods provide a means to address this gap in knowledge by monitoring the conformational status of proteins within cells at the proteome-wide scale. Here, we demonstrate the ability of a protein painting method employing tetraphenylethene maleimide (TPE-MI) to reveal proteome network remodeling in whole cells in response to a cohort of commonly used pharmacological stimuli of varying specificity. We report specific, albeit heterogeneous, responses to individual stimuli that coalesce on a conserved set of core cellular machineries. This work expands our understanding of proteome conformational remodeling in response to cellular stimuli, and provides a blueprint for assessing how these conformational changes may contribute to disorders characterized by proteostasis imbalance. |
Databáze: |
Directory of Open Access Journals |
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