A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay

Autor: Anju Mohan, Hari Mohan Saxena, Puneet Malhotra
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Veterinary World, Vol 9, Iss 7, Pp 717-722 (2016)
Druh dokumentu: article
ISSN: 0972-8988
2231-0916
DOI: 10.14202/vetworld.2016.717-722
Popis: Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols. Results: The mean STAT titers were 1.963±0.345 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was extremely significant (p
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