| Autor: |
Ilaria Gandolfini, Cynthia Harris, Michael Abecassis, Lisa Anderson, Oriol Bestard, Giorgia Comai, Paolo Cravedi, Elena Cremaschi, J. Andrew Duty, Sander Florman, John Friedewald, Gaetano La Manna, Umberto Maggiore, Thomas Moran, Giovanni Piotti, Carolina Purroy, Marta Jarque, Vinay Nair, Ron Shapiro, Jessica Reid-Adam, Peter S. Heeger |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Kidney International Reports, Vol 2, Iss 6, Pp 1186-1193 (2017) |
| Druh dokumentu: |
article |
| ISSN: |
2468-0249 |
| DOI: |
10.1016/j.ekir.2017.06.010 |
| Popis: |
Measuring the chemokine CXCL9 in urine by enzyme-linked immunosorbent assay (ELISA) can diagnose acute cellular rejection (ACR) noninvasively after kidney transplantation, but the required 12- to 24-hour turnaround time is not ideal for rapid, clinical decision-making. Methods: We developed a biolayer interferometry (BLI)−based assay to rapidly measure urinary CXCL9 in 200 pg/ml in subjects with ACR and ≤100 pg/ml in subjects with stable kidney function without cellular infiltrates. In samples obtained after treatment for ACR, BLI CXCL9 measurements detected biopsy-proven intragraft infiltrates despite treatment-induced reduction in serum creatinine. Discussion: Together, our proof-of-principle results demonstrate that BLI-based urinary CXCL9 detection has potential as a point-of-care noninvasive biomarker to diagnose and guide therapy for ACR in kidney transplantation recipients. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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