| Popis: |
The study of fruit development in zucchini via gene expression has proven to be applicable in breeding programs. Phenotypic and transcriptomic studies of fruit set and parthenocarpy have been previously developed and some relevant genes have been reported. From these studies, three genotypes (MUCU-16, Whitaker, and Cavili) and six genes (CpAUX22, CpIAA4, CpIAMT-1, CpPIN5, CpCYCD6-1, and CpEXPLB1) were selected. The expression of these genes was analyzed in each genotype under three different treatments (pollination, auxin-treatment and non-treatment) during one week post anthesis. Also, a phenotyping analysis was conducted. The different nature of the samples and the genes selected allowed associations between different fruit traits and fruit development stages. There was a rapid response of CpAUX22 and CpIAA4 to the auxin treatment. Also, these genes and the CpIAMT-1 became more overexpressed in pollinated samples over time. The CpPIN5 gene increased its expression over time in all genotypes while CpCYCD6-1 was overexpressed in the early stages of fruit development in all samples. The CpEXPLB1 was highly up-regulated in non-treated samples, suggesting a relationship with fruit abortion. The overexpression of CpAUX22 and the non-overexpression of CpEXPLB1 in early stages may be associated with fruit growth in zucchini. |
